Immunostaining of PNMT and peripherin in adrenal medulla.

The expressions of PNMT and peripherin in the adrenal medulla of each group were detected by immunohistochemistry. A representative sample of PNMT immunostaining (DAB, 100×) was presented for the control rats (A), s-control rats (B), the asthmatic rats (C), and the s-asthmatic rats (D). A representative peripherin immunostaining (DAB, 100×) was presented for the control rats (E), s-control rats (F), the asthmatic rats (G), and the s-asthmatic rats (H). Arrows indicate the peripherin-positive ganglion cells. Bar graphs demonstrate the average optical density of PNMT (I) and peripherin (J) and the number of peripherin-positive ganglion cells (K). The values were presented as ±s (n = 8). * indicates significant difference as compared with the control group (P<0.05); # indicates significant difference between the s-asthma and the asthma groups (P<0.05).