Transcriptome analysis of embryonic axes of soybean seeds germinating in the presence of the gibberellin (GA) biosynthesis inhibitor paclobutrazol (PBZ). Transcriptome analysis of embryonic axes of soybean seeds germinating in the presence of the gibberellin (GA) biosynthesis inhibitor paclobutrazol (PBZ)

Purpose: A time-course transcriptome study to identify probable GA-responsive genes in soybean embryonic axes during seed germination. Methods: Seeds were germinated in the presence or absence of 200 µM PBZ. Seeds were germinated in 28°C temperature and 12/12h photoperiod (dark/light) and harvested at 12, 24 and 36 hours after imbibition (HAI). Three biological replicates were performed. Results: Identification of GA-responsive genes during germination in Glycine max. Overall design: RNA-Seq analysis of embryonic axes of soybean seeds at 12, 24 and 36 hours after imbibition in the presence of paclobutrazol, a gibberellin biosynthesis inhibitor.