Primers for genome cloning and mutagenesis of IBDV.^a

aThe positions where the primers GtAG876AU, GtAG876AL, GtAG896AU, and GtAG896AL bind are in accordance with the published sequence of IBDV strain Gt (GenBank assession nos: DQ403248). The positions where other primers bind are in accordance with the published sequence of strain Gx (GenBank assession nos: AY444873 and AY705393). The introduced mutations are lower-case characters. Orientation and position of the primers are shown.