Atlas of FSHR expression from novel reporter mice

The FSH-FSHR signaling pathway has traditionally been considered an essential regulator in reproductive development and fertility. But there has been emerging evidence of FSHR expression in extragonadal tissues/organs. This poses new questions and long-term debates regarding the physiological role of the FSH-FSHR pathway, and underscores the need for reliable, in vivo analysis of FSHR expression in animal models. However, conventional methods have proven insufficient for examining FSHR expression due to limitations, such as the scarcity of ‘reliable’ antibodies, rapid turnover/degradation of transcripts, and a lack of robust in vivo tools. To address this challenge, we developed Fshr-ZsGreen ‘knockin’ reporter mice under the control of Fshr endogenous promoter using CRISPR/Cas9 genome-editing technology to append a P2A-ZsGreen targeting vector into a locus between the last exon and the stop codon of Fshr. With this novel genetic tool, we provide a reliable readout of Fshr expression at ..., This dataset includes raw data collected by images of confocal microscope by IF and single RNA molecule-fluorescence in situ hybridization and dd.rtPPCR, etc., which were described in more details in Methods of our publication., , # Atlas of FSHR expression from novel reporter mice

[https://doi.org/10.5061/dryad.1jwstqk4t](https://doi.org/10.5061/dryad.1jwstqk4t)

## Description of the data and file structure

This dataset includes raw data for gel images and microscope images for data published in our manuscript at the eLife, which are compressed and can be depressed with ZIP.

The submitted files are in two forms:

1\) *TIFF images can be viewed by Photoshop or Image J;

2\) *xlsx files can open the Eslsx file.

## The description of these data:

## **1. Fig. 1 B and C images for genotyping PCR and Southern blots: 2-sub-folders**

### (1) Fig. 1B PCR images for genotyping-4 images in TIF Fig. 1B-b and c 

Two primer pairs were used for genotyping heterozygote mice to produce the following two images: 

1\)  Uncropped image for Fig. 1B-b;

2\)  Uncropped image for Fig. 1B-c;

3\) Labelled images for Figure1B-b;

4\)  Labelled images for Figure1B-c.

### (2) Fig. 1C images for Southern blots-6 TIFF images

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