SpaceX Inspiration4 PBMC Profiling: snRNA-seq, snATAC-seq, and T-cell and B-cell repertoire profiling

The SpaceX Inspiration4 mission was a 3-day mission with four private astronauts to low Earth orbit that occurred in September 2021. The crew collected biospecimen samples before and after flight. One of these biospecimen collections included PBMCs collected via venipuncture two 4mL K2 EDTA tubes (BD Biosciences, cat# 367844), collected pre-flight (L-92, L-44, L-3) and post-flight (R+1, R+45, R+82). Single-nuclei RNA-seq and single-nuclei ATAC-seq were co-assayed using the 10X Genomics Multiome Kit (L-92, L-44, L-3, R+1, R+45, R+82 timepoints). T-cell and B-cell V(D)J repertoires were profiled using the 10X Genomics Chromium Next GEM Single Cell 5’ Kit (L-3, R+1, R+45, R+82 timepoints). Libraries were sequenced on the Illumina platform. Differential gene expression profiles and clonotypes detected per timepoint were calculated from sequencing results.