Human Skeletal Stem Cell Diversity

We have used SmartSeq2 to sequence single phenotypic human skeletal stem cell (SSCs) and downstream lineage populations purified via FACS from fetal skeletal tissues and patient specimens. SSC populations were isolated based on their previously reported surface marker profiles. A human SSC (CD45-CD235a-CD31-TIE2-CD146-PDPN+CD73+CD164+), a human bone-cartilage-stroma-progenitor (BCSP; CD45-CD235a-CD31-TIE2-CD146+PDPN+) and an osteoprogenitor (OP; CD45-CD235a-CD31-TIE2-CD146+PDPN-) were investigated. Overall design: SmartSeq2 single cell RNA sequencing of freshly, prospectively, flow cytometrically purified single skeletal stem cell and lineage population from human fetal tissue (17-20 weeks gestational age) and patient specimens (18-77 years of age).