EDC exposure during SC-islet differentiation

We aimed to asses how EDC exposure in combination with a genetic predisposition for diabetes could impact pancreatic endocrine cell development. For this, we used a protocol to generate Stem-cell islets (SC-islets) from induced pluripotent stem cells (iPSCs) following a 7 satege protocol. For 2 weeks after pancreatic endocrine progenitor stage, cells were treated with a EDC mix of 1 nm of Bisphenol A, bisphenol S, and trans-nonachlor, or the vehicle DMSO. After those 2 weeks, the cells got a 1 week recovery period before collection. In the present study we used both iPSCs carrying the P291fsinC mutation in the HNF1A gene, and and isogenic non carrier control.