Development of gene expression signatures for BV-2 microglia activation in the background of mild hypothermia

Sepsis associated encephalopathy (SAE), a common complication of sepsis, seriously affect the prognosis and quality of life to sepsis patients. Microglia activation is vital to the neuroinflammation and the pathology of SAE. Mild hypothermia, that a useful procedure in the treatment of traumatic brain injuries, was found to suppress microglia activation. In present study, in vitro cultured BV-2 microglial cells stimulated with LPS was employed as the model of microglia activation. The altered signitures of lncRNAs, circRNAs and mRNAs of LPS exposure and mild hypothermia were arrayed by using the Agilent ceRNA Microarray Chip. BV-2 cells were divided into four group: mild hypothermia (33◦C), mild hypothermia (33◦C) and lipopolysaccharide (1000ng/ml), normal temperature (37◦C), normal temperature (37◦C) and lipopolysaccharide(1000ng/ml). The experiment repeated for three time.