Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 3' terminal phosphates and crosslinked with EDC.

Protein Data Bank Japan2024-11-06 更新2026-04-25 收录

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Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 3' terminal phosphates and crosslinked with EDC. Descriptor: DNA (5'-D(*CP*CP*CP*GP*GP*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*AP*CP*GP*GP*A)-3', DNA (5'-D(*CP*CP*GP*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP*GP*GP*A)-3'), MAGNESIUM ION, ... Authors: Orun, A.R, Shields, E.T, Shrestha, R, Slaughter, C.K, Snow, C.D. Deposit date: 2023-07-20 Release date: 2023-08-02 Last modified: 2024-11-06 Method: X-RAY DIFFRACTION (3.11 Å) Cite: Tuning Chemical DNA Ligation within DNA Crystals and Protein-DNA Cocrystals. Acs Nanosci Au, 4, 2024

本数据集为同网状互穿共晶体，组分包含复制起始蛋白（Replication Initiator Protein）REPE54与对称扩增31聚体双链体；该双链体携带有同源REPE54序列及额外富G-C序列，具备平末端与3'端磷酸基团，并经EDC交联。描述符：脱氧核糖核酸（DNA，5'-D(*CP*CP*CP*GP*GP*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*AP*CP*GP*GP*A)-3'）、脱氧核糖核酸（DNA，5'-D(*CP*CP*GP*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP*GP*GP*A)-3'）、镁离子（MAGNESIUM ION），…… 作者：Orun, A.R、Shields, E.T、Shrestha, R、Slaughter, C.K、Snow, C.D 入库日期：2023年7月20日发布日期：2023年8月2日最后修改日期：2024年11月6日实验方法：X射线衍射（3.11埃）引用文献：《调控DNA晶体与蛋白-DNA共晶体中的化学DNA连接》（Tuning Chemical DNA Ligation within DNA Crystals and Protein-DNA Cocrystals），《ACS Nanosci Au》，第4卷，2024年

创建时间：

2023-07-20