Supplementary data: An improved gene synthesis method with asymmetric directions of oligonucleotides designed based on a simulation program

Table
S1 Name, length and nucleotide sequence of oligonucleotide
used in AESOE reaction

Table S2 Integer-number pairs simulating annealing
and extension reaction of ssDNA

Figure S1 Gel electrophoresis of AESOE-synthesized
DNA-fragments Five μl of the
AESOE reaction solution taken out after the cycle shown on the photograph was
analyzed by 1.5% agarose electrophoresis. M: Size marker (100, 200, 300, 400
and 500 bases from the bottom). The name of the oligomer set is shown on the
left of the photograph.

Figure S3
Schematic diagram of the incomplete dimer from which an extension reaction is
initiated. The direction of the arrow indicates the direction of the base
sequence of the oligomer (5’->3’).

Figure S4
Schematic diagram of the dimers from which any extension reaction is not
initiated. The direction of the arrow indicates the direction of the base
sequence of the oligomer (5’->3’).

Figure S5
Schematic diagram of tendency on reconstruction of original duplex of DNA
comparing incomplete dimer formation. The direction of the arrow indicates
the direction of the base sequence of the oligomer (5’->3’). Black and red
arrows indicate original duplex DNA and short oligomer respectively.

Figure S6 Schematic
diagram of tendency on dimer formation. Possibility of hybridization with
short oligomers is higher than with long oligomers. The direction of the
arrow indicates the direction of the base sequence of the oligomer
(5’->3’).

Figure S7
Schematic diagram of tendency on dimer formation. Possibility of
hybridization with oligomers present in high concentration is higher than
those in low concentration. The direction of the arrow indicates the
direction of the base sequence of the oligomer (5’->3’).

Supplement methods. Simulator for Oligomer
Polymerization