TDP-43 regulates miRNA biogenesis and cytotoxicity by obstructing Dicer activity

The cytoplasmic Dicer proteins play a critical role in miRNA biogenesis. Deficiency of TDP-43 fly homologs, TBPH, and expression of its A315T pathogenic mutations reduces the levels of Drosophila dicer proteins (DCR-1 and DCR-2) in ALS fly models. Figure 1A and Figure 5A contain the original image files of Western Blot that were used to evaluate the expression levels of miRNA biogenesis-related proteins (including DCR-1 and DCR-2) in TBPH KO (compare to W- and heterozygous, Figure 1A) and TBPH A315T mutant (compare to TBPH WT and LacZ control, Figure 5A), flies. The files "W-vs-TBPHdelta23-mRNA.filter.annot.xls.zip" and "W-vs-TBPHdelta23-miRNA.filter.xls.zip" contain the miRNA and RNA reference sequencing data, including annotations and expression levels, from TBPH KO and W- control flies.