Invasive lobular carcinoma (ILC) is the second most common breast cancer subtype, accounting for 8-14% of all cases. While the majority of human ILCs are characterized by the functional loss of E-cadh
Transposon library construction, genomic DNA Extraction, and sequencing. Transposon library after buoyancy centrifugation was collected and resuspended in 400ul 10mM Tris (pH=9). After beads-be
The goal of this project is to leverage Quantitative Insertion-site Sequencing (QIseq) methods, an Illumina sequencing approach to identify piggyBac transposon insertion sites, to experimentally ident
NT—Not tested.*950 mutants screened.Number of selected Pseudomonas syringae pv. phaseolicola transposon disruption mutants from 960 screened for each phenotype.
