32 Kinase-Inhibitor Perturbed RNA sequencing of 11 cancer cell-lines

The goal of the CTD2 Pancancer Drug Activity DREAM Challenge is to foster the development and benchmarking of algorithms to predict targets of chemotherapeutic compounds from post-treatment transcriptional data. The drug perturbational profiles on 11 cell lines for 32 kinase inhibitors with well-established targets were provided to challenge participants, without revealing the identity of the drugs. These profiles were used to predict the drug-targets of the 32 anonymized drugs using publically available datasets for training. Overall design: 1728 RNAseq profiles of a cell-line perturbed with 32 kinase inhibitors. Cell-lines were treated at a 48-hour IC20 for each drug and RNA was collected at 24 hours to minimize cell-death. Samples were prepared and sequences in batches of 96 that included 6 vehicle (DMSO) and 6 water controls. 2 replicates were taken for each drug-cell-line pair.