Circulating microRNA as potential diagnostic biomarkers for sleep disorder

Next Generation Sequencing (NGS) was carried out on subjects who visited an oriental medicine clinic due to recurrent sleep disturbance, and 40 individuals (17 male and 23 female) were set as the sleep diorder (SD) group based on their scores on the sleep distrurbance questionnaire of Pittsburgh sleep quality index (PSQI). The control group consisted of 40 healthy individuals (20 males and females each), as assessed by both subjective diagnosis and test for metabolic syndrome factors.Ten individuals (five males and females each) from the SD and the control group, respectively, were allocated for NGS analysis. Overall design: Circulating miRNA profile in patient serum samples for identifcation of potential biomarkers in sleep disorder (sleep control: n=10, sleep disorder: n=10)