Locomotor behavior summary for <i>c929-Gal4-</i>mediated RNAi knockdown of PDF.

c929-Gal4-driven expression of uas-Dcr2 appeared to reduce DD rhythmicity (see values for y w,uas-Dcr2/y;; in Table 1), although the majority of these flies maintained free-running locomotor rhythms. Loss of PDF from the l-vLNs and s-Ab PDF neurons in y w,uas-Dcr2/y;c929-Gal4/+;uas-PDFrnai/+flies was not associated with a significant change of τ relative to controls. Although ANOVA revealed significant differences in τ between genotypes (p = 0.0023), a Tukey's test revealed no significant difference between y w,uas-Dcr2/y;c929-Gal4/+;uas-PDFrnai/+ flies and y w,uas-Dcr2/y;;uas-PDFrnai/+ controls. ANOVA also revealed significant differences in power between genotypes, but a Tukey's test revealed no significant difference between y w,uas-Dcr2/y;c929-Gal4/+;uas-PDFrnai/+ flies and y w,uas-Dcr2/y;;uas-PDFrnai/+ controls. Similarly, ANOVA revealed significant differences in evening peak phase between genotypes (p<0.0001), but a Tukey's test revealed that y w,uas-Dcr2/y;c929-Gal4/+;uas-PDFrnai/+flies did not differ significantly from controls (p>0.05 for each pair-wise comparison).