Global subcutaneous white adipose tissue (scWAT) transcript data from fasted male BXD strains on chow or high fat diet

Transcript data from subcutaneous white adipose tissue (scWAT) from fasted-state male BXD strains on chow or high fat diet We used microarrays to compare the subcutaneous white adipose tissue (scWAT) expression differences across the BXD strain family and across two diverse diets 29-week-old male mice were fasted overnight (6pm-9am), anesthetized under isoflurane, and perfused. After about five minutes (towards the end of the tissue collection pipeline), subcutaneous white adipose tissue was weighed then snap-frozen in liquid nitrogen. Later, the tissue was taken from a -80 freezer for RNA extraction from all individuals. Each dietary and strain cohort consisted of ~5 animals which were prepared independently. These independent RNA samples were then then pooled into single dietary and genetic cohorts (as shown here) evenly by µg RNA as measured by Nanodrop. These pools were cleaned up with RNEasy, then frozen again in -80. Samples were then shipped from Lausanne, Switzerland to Memphis, TN, where the Affymetrix arrays were run. Tissues were in the -80 freezer approximately 1-2 years before RNA was prepared. RNA (post pooling by cohort, but pre-RNEasy cleanup) were in the freezer approximately 2.5 years before microarrays were run.本数据集为普通饲料或高脂饮食条件下处于禁食状态的雄性BXD品系小鼠皮下白色脂肪组织（subcutaneous white adipose tissue, scWAT）的转录组数据。本研究利用微阵列技术，比较了BXD品系小鼠家族在两种不同饮食条件下的scWAT基因表达差异。实验对象为29周龄雄性小鼠，于晚间6点至次日上午9点进行整夜禁食，随后用异氟烷进行麻醉并实施灌注。在组织收集流程接近尾声时（灌注后约5分钟），采集皮下白色脂肪组织并称重，随后置于液氮中快速冷冻。后续从-80℃冰箱中取出所有小鼠的组织样本，用于RNA提取。每个饮食和品系队列包含约5只独立制备的小鼠。将这些独立提取的RNA样本按照Nanodrop检测的微克数均匀混合，分别归入对应的饮食与遗传队列（如本图所示）。混合后的RNA样本使用RNeasy试剂盒进行纯化，随后再次置于-80℃冰箱中保存。随后将样本从瑞士洛桑运往美国田纳西州孟菲斯市，在当地进行Affymetrix微阵列实验。组织样本在-80℃冰箱中保存约1-2年后才进行RNA提取；而队列混合后、RNeasy纯化前的RNA样本则在冰箱中保存约2.5年后才进行微阵列实验。