In vitro evidence consistent with an interaction between wild type and a mutant SOD1 protein associated with canine degenerative myelopathy

The data sets are associated with four experimental approaches to investigate a mutant gene that had been cloned into a vector plasmid to generate fluorescent tagged (Cherry or GFP wildtype or mutant SOD1 proteins: a. a collection of fluorescent images of cells to count aggregate positive cells (WT and Mutant (DM) with GFP or Cherry) (JPEGS format) b. A collection of western blots that confirm the expression of the SOD1 fusion protein, the fluorescent protein only and loading control with b-actin. (Tiff format) c. A native gel treated with NBT to display SOD1 activity. Corresponding western blots shown to confirm SOD1 fusion protein expression. (Tiff format) d. Confocal images showing the co-expression of wild type and mutant fusion proteins within cellular aggregates. (Tiff format) e. Western blot of immunoprecipitation (pull down) products supporting the co-precipitation of wildtype and mutant SOD1. (Tiff format)