RNA-seq Analysis on the Renal Tissue Injury in the Development of Goslings Gout

The transcription expressions of gout goslings in renal tissues was significantly different from that of healthy goslings. With P <0.05 and |log2(fold change)| >1 as the selection standards, 1749 differential expression genes were found. Among these, 501 genes were up-regulated and 1248 genes were down-regulated in the gout group. The gene ontology (GO) search revealed many significant terms, including chemokine activity, T cell receptor binding, antigen binding, immune response, G-protein coupled receptor signaling pathway, chemokine-mediated signaling pathway, inflammatory response and so on. The Kyoto Encyclopedia of Genes and Genomes (KEGG) selected the cytokine-cytokine receptor interaction, intestinal immune network for IgA production, cell adhesion molecules. In conclusion, the kidney injuries in gout goslings is closely related to immune response, inflammatory response and cell adhesion processes. Overall design: We used RNA-seq to measure the transcription differences in renal tissues between gout and healthy goslings during their disease period. Five goslings with typical visceral gout characteristics and serum manifestations were selected as “gout group”, and six healthy goslings were also selected as the “control group”. RNA-seq was used to test the transcription differences in renal tissues between gout and control groups.