Conditions for FT-derived neurosphere differentiation.

Seven different conditions involving various combinations of an adhesive substrate with serum-containing medium were used to differentiate FT-derived neurospheres. After 7 days, the differentiated cells were subsequently immunostained for markers to identify neurons (βTIII, neurofilament), astrocytes (GFAP), and oligodendrocytes (O1). Although all seven conditions generated these cell types, exposing neurospheres to serum resulted in a more rapid differentiation process. N.D.: not done.