Targeting APEX2 to the mRNA encoding fatty acid synthase β in yeast identifies proteins that bind and control its translational efficiency in the cell cycle

To understand how the translation of FAS1, encoding fatty acid synthase, peaks late in the cell cycle, we identified proteins that bind the FAS1 transcript in a cell cycle-dependent manner. We used dCas13d-APEX2 fusions to target FAS1 and label nearby proteins, which were then identified by mass spectrometry.