Mass spectrometry-based draft of the mouse proteome

Quantitative draft map of the proteomes of 41 healthy tissues of the animal model Mus musculus and a panel of 66 murine pancreatic ductal adenocarcinoma cell lines and. Our in-depth analysis provides evidence for proteins encoded by >16,000 genes (~76% of the total annotated protein-coding genes), where they are expressed and in which quantities. Moreover, a widespread yet tissue- and cell type-specific phosphorylation pattern is clearly represented in our data (>50,000 sites). We further leverage our analysis by integrating the phenotypic response of a the murine PDAC cell lines panel to cancer drugs and ionizing radiation, to shed light on the molecular mechanisms underlying drug action and radioresistance. This large and unique atlas is made available to the scientific community also via ProteomicsDB (https://www.proteomicsDB.org) and the interactive web application PACiFIC (http://pacific.proteomics.wzw.tum.de).