untargeted metabolomics analysis of fecal extract after resveratrol treatment

In a previous study, we found that resveratrol could inhibit chylomicron secretion and jejunal SR-B1 expression, and the effects were mediated by gut microbiota and some heat-stable metabolites in the feces of mice which received resveratrol treatment. We then conducted metabolomics profiling of fecal extract (FE) from mice receiving 8-week high-fat diet (HFD) or HFD plus resveratrol (0.5% in diet) treatment. HFR-FE was also heated at 95℃ for 5 min, referring to the HRH-FE group. Through LC-MS/MS analysis, we detected 2053 metabolites. Principal component analysis (PCA) revealed that composition of metabolites in HFD-FE and HFR-FE are different, and after heat treatment, a large portion of metabolites in HFR-FE was transformed. We then conducted differential analysis and identified 238 differential metabolites between HFR and HFD, and 432 differential metabolites between HRH and HFD. The KEGG pathway enrichment analysis suggested that sphingolipid metabolism and bile acid metabolism-related pathways were mostly modulated by resveratrol treatment.