Fully human umbilical cord-derived organoid for acute liver failure

We report a fully human UC-derived organoid system that integrates ECM hydrogel, MSCs and reprogrammed hepatocytes, achieving the treatment of acute hepatic failure in case of extreme failure.To explore the mechanism of our organoid in hepatic failure, we performed single cell RNA sequencing of transplanted organoids as well as the original organoid prior to transplantation, we have demonstrated that MSCs were primarily responsible for inhibiting the secretion of inflammatory-inducing cytokines and promoting the secretion of inflammation-inhibiting cytokines by recruiting immune cells migrating into organoid to triggering immune response. Overall design: single-cell RNA-seq of transplanted organoids (including both human MSCs and rat cells) on day 3 and 10, as well as the original organoid (only human MSCs) prior to transplantation.