Transcriptomic analysis of plasma extracellular vesicles from patients with ischemic stroke of different etiologies undergoing mechanical thrombectomy. Transcriptomic analysis of plasma extracellular vesicles from patients with ischemic stroke of different etiologies undergoing mechanical thrombectomy

INTRODUCTION: Accurate ischemic stroke (IS) etiologic diagnosis provides an appropriate secondary prevention strategy and better outcome. Currently, 45% of IS are cryptogenic urging to find novel approaches to enhance diagnostic precision and secondary stroke prevention. OBJETIVE: To study the transcriptomic content of circulating extracellular vesicles (EVs) to delineate the molecular profile and potential biomarkers of IS etiologies. RESULTS:21 consecutive patients with moderate or severe ischemic stroke (IS) and different IS etiologies were included to perform the transcriptomic analysis of plasma EVs comprising: atherothrombotic (AT, n=6), Cardioembolic (CE, n=10), and embolic stroke of undetermined source (ESUS, n=5). The RNASeq analysis of EVs detected clusters of differentially expressed genes (DEGs) related to IS etiologies. Overall design: Transcriptome of patients with ischemic stroke of atherothrombotic (AT, n=6), cardioembolic (CE, n=10) or undetermined etiology (ESUS, n=5) were compared in circulating extracellular vesicles .