Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE [gRNA sequencing for ABE targets]

To identify potential contaminating gRNAs, we adapted a gRNA sequencing assay based on an established SMARTer smRNA sequencing technology. We evaluated five research-grade gRNAs from three synthetic gRNA manufacturer suppliers (A, B and C). We detected the presence of contaminating gRNAs in CBLB and CIITA gRNAs from supplier A (batch 1), with frequency ranging from 0.09% to 2.71%. Overall design: guide-RNA (gRNA) sequencing of commerically synthesized gRNAs from multiple manufacturers (suppliers A, B, and C) using modified SMARTer smRNA-seq technology.