The profile of miRNAs secreted into the supernatant during cardiac differentiation and maturation from human induced pluripotent stem cells verified by miRNA array analysis.

We performed miRNA array analysis to analyze the miRNAs secreted into the supernatant during the mesoderm/cardiac differentiation and maturation process from human induced pluripotent stem cells (hiPSCs). Supernatant samples were collected on day -1, day 3, day 5, day 7, day 9, day 21, day 35, and day 51. We conducted mesoderm and cardiac differentiation from hiPSCs(253G4) and cultured over the long term and supernatant samples were collected on day -1, day 3, day 5, day 7, day 9, day 21, day 35, and day 51 after the initiation of differentiation induction. miRNA was isolated from cell culture supernatant samples and subsequently we performed microarray analysis.