Genome-wide binding of FRUITFULL complexes (FUL-SOC1, FUL-AG-SEP3, FUL, FUL-AG, FUL-SEP3) determined by DNA-affinity purification sequencing (DAP-seq)

With this experiment, our objective is to identify the binding sites of MADS-domain protein complexes and determine if they exhibit binding to different targets or with differing affinities, which could be a mechanism to acquire target gene specificity. Specifically, we aim to investigate this phenomenon for the tetramers FUL-SOC1 and FUL-AG-SEP3, as they are enriched in different tissues, thus likely regulating distinct functions. FUL-SOC1 is enriched in the inflorescence meristem, whereas the FUL-AG-SEP3 complex is enriched in the siliques. Using the DAP-seq protocol, we established their genome-wide binding profiles and included other FUL complexes (FUL, FUL-AG, and FUL-SEP3) as controls. Overall design: We use an in vitro genome-wide DNA binding assay, known as DAP-seq, to determine the genome-wide binding patterns of FUL-SOC1, FUL-AG-SEP3, FUL, FUL-AG, and FUL-SEP3.