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Rates of hydrolysis of physiological peptides by NEP mutants.

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https://figshare.com/articles/dataset/_Rates_of_hydrolysis_of_physiological_peptides_by_NEP_mutants_/348279
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Hydrolysis was carried out at 37°C at in 20 mM MES buffer, pH 6.5. Substrate concentrations were 15 µM insulin B chain, 24 µM Aß1–40, and 64 µM leu-ENK. Activity was determined by following the disappearance of substrate by HPLC. Each reaction was run in at least triplicate. Statistical analysis was conducted using a two-tailed paired t-test with Prism4 software.
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2015-12-02
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