Pharmacological characterization of MT<sub>1</sub> and MT<sub>2</sub> receptor mutants.
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Kd values for 125I-MLT were determined in radioligand saturation experiments with increasing concentration of 125I-MLT. IC50 values for melatonin were determine in competition bindings experiments with 200 pM of 125I-MLT. Ki values were calculated from IC50 values using the Cheng-Prussof formula Ki = IC50/(1+[L]/Kd. Experiments were repeated 3–7 times (*, P<0.05; **, P<0.01; ***, P<0.001 vs wt).
创建时间：
2010-07-15
