Protein interactions of transcriptional co-regulator LEUNIG_HOMOLOG (LUH) during early stages of Arabidopsis flower development.

To study the formation and dynamics of LUH protein-protein interactions at various stages of flower development, we conducted a series of immunoprecipitation followed by mass spectrometry (IP-MS) experiments using the LUH-GFP in floral induction system (FIS) plant line (pLUH:LUH-GFP pAP1:AP1-GR ap1 cal). We focused on four time points after the induction of flowering corresponding to: undifferentiated inflorescence meristem (0 days after induction, 0 DAI), meristem specification (2 DAI), organ specification (4 DAI), and organ differentiation (8 DAI).