Investigating the Impact of Acinetobacter lwoffii-ECT1/E6E7 Co-culture Metabolites on Cervical Cell Proliferation and Apoptosis via Untargeted Metabolomics.

This study employed untargeted metabolomics detection combined with bioinformatics analysis to investigate the metabolites from the co-culture of Acinetobacter lwoffiiand cervical precancerous cells ECT1/E6E7. A. lwoffiiwas co-cultured with ECT1/E6E7 cells at an MOI of 100. The culture supernatants from six groups were collected: ECT1/E6E7 cells cultured alone for 18h (E_18h) and 30h (E_30h), A. lwoffiicultured alone for 18h (L_18h) and 30h (L_30h), and the co-culture of ECT1/E6E7 cells with A. lwoffiifor 18h (E_L_18h) and 30h (E_L_30h). Broad untargeted metabolomics technology was used to detect the differential metabolites in the co-culture. The untargeted metabolomics results showed that the most significant differential metabolites at the intersection of the L group, E group, and E_L group at 18h and 30h of co-culture were gluconic acid and p-Cresol, respectively.