Images of selected cells of the TbGOS02 recombinant Trypanosoma brucei brucei cell line

A novel experimental setup for measuring fluorescence in live trypanosome (FILT) cells was established as described in Borg (2015) and Borg et al. (2021), a study which will be linked to this dataset upon publication. The FILT set up was used to capture 60 fluorescent and 60 brightfield images of a field of vision which encompassed 88 cells of the recombinant Trypanosoma brucei brucei cell line, TbGOS02. All these images are provided in the file within this dataset labelled, "TbGOS02 All Frames 88 cells 140619.pdf".

Each of the 88 cells in the image was enumerated as detailed in Borg et al. (2021). For cell number 08, 60 fluorescent and 60 brightfield cropped images featuring only this cell are provided in the file within this dataset labelled, " TbGOS02 All Frames Cell 08 140619.pdf ". Equivalent images for cells numbered 17, 63 and 76 are provided in the remaining three PDF files of this dateset, as summarised below.

TbGOS02 All Frames 88 cells 140619.pdf
All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of the 88 cells in view field.

TbGOS02 All Frames Cell 08 140619.pdf
All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of cell 08.

TbGOS02 All Frames Cell 17 140619.pdf
All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of cell 17

TbGOS02 All Frames Cell 63 140619.pdf
All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of cell 63

TbGOS02 All Frames Cell 76 140619.pdf
All 120 captured images captured in the FILT setup, 60 fluorescent and 60 brightfield, of cell 76

References
Borg Y (2015). Design, Mathematical Modelling, Construction and Testing of Synthetic Gene Network Oscillators to Establish Roseobacter Clade Bacteria and the Protozoan Trypanosoma brucei as Synthetic Biology Chassis. Doctoral thesis, University College London. https://discovery.ucl.ac.uk/id/eprint/1472672

Borg Y, Alsford S, Pavlika V, Zaikin A, Nesbeth D (2021). Synthetic biology tools for engineering Goodwin oscillation in Trypanosoma brucei brucei. In preparation.