Sanger sequence chromatograms of 17KDa and gltA Rickettsia genes demonstrating double peaks

A multilocus sequencing study of Torix group Rickettsia in arthropods providing evidence of co-infection with Rickettsia. During Sanger chromatogram analysis, double peaks were occasionally found at third codon sites from protein coding genes. This pattern was observed in 6/10 Philotarsus californicus individuals and in one member of each of the Psilidae, Sciaridae, Chironomidae and Diapriidae. Where double peaks were observed, this was found consistently across markers within an individual specimen. This pattern indicates co-infecting Rickettsia strains in hosts is a widespread phenomenon of the Torix group.一项针对节肢动物体内Torix群立克次体（Torix group Rickettsia）的多位点测序研究，为其与立克次体的共感染现象提供了实验佐证。在桑格色谱（Sanger chromatogram）分析环节，研究人员偶于蛋白质编码基因的密码子第三位观测到双峰信号。该现象见于10头加州毛柱跳虫（Philotarsus californicus）个体中的6头，以及斑蝇科（Psilidae）、眼蕈蚊科（Sciaridae）、摇蚊科（Chironomidae）与广腹细蜂科（Diapriidae）各1头样本。在观测到双峰信号的个体中，该特征在其所有检测标记基因中均稳定一致。该结果表明，宿主体内存在共感染的立克次体菌株，是Torix群立克次体的一类普遍现象。