Whole-genome sequencing of the <i>Streptomyces coelicolor bldA39</i> mutant (J1700) of reveals hundreds of previously unknown mutations.

<b>Abstract</b>We report the genome sequence of the <i>bldA39 </i>(J1700) mutant <i>Streptomyces coelicolor</i>, a historically important strain that is deficient in sporulation and antimicrobial production. The <i>S. coelicolor</i> J1700 strain was used extensively from the 1980s onwards to underpin important discoveries in development and antibiotic production in <i>Streptomyces.</i> The <i>bldA </i>gene encodes a leucyl transfer RNA (tRNA), required for the translation of the rare TTA codon found in ~2 % of genes<i> </i>in <i>Streptomyces. </i>The whole genome of <i>S. coelicolor</i> J1700 was obtained via Illumina sequencing and mapped to the of <i>S. coelicolor </i>M145 reference genome. Analysis of the genome sequence compared to <i>S. coelicolor</i> M145 identified the known <i>bldA39</i> mutation (T&gt;C) and revealed more than 300 further mutations, likely associated with the <i>S. coelicolor </i>J1501 genetic background the strain was created in, including the nature of the <i>hisA1 </i>and <i>uraA1</i> alleles used extensively in genetic mapping experiments and several mutations in natural product biosynthetic gene clusters. This work highlights the importance of whole genome sequencing of historically important strains.<br><b>Data Summary</b>This Whole Genome Sequencing project has been deposited in NCBI under the Bioproject PRJNA1186139. The WGS reads used can be accessed in the NCBI’s SRA under the accession number SAMN44744323.