Gene expression analysis following 6h-, 12h-, 18h- and 24h-siRNA knockdown of transforming growth factor β receptor III (TGFBR3) in human pulmonary arterial smooth muscle cells (hPASMC).. Homo sapiens

We explored the impact of transforming growth factor β receptor III (TGFBR3) knockdown on the gene expression in the human pulmonary arterial smooth muscle cells (hPASMC), following 6h, 12h, 18h and 24h. Overall design: Human pulmonary arterial smooth muscle cells (hPASMC) in P4 were plated in 6-well plates and transfected with either 200nM siRNA to transforming growth factor β receptor III (TGFBR3) (Santa Cruz Biotechnology) or Scrambled control (Ambion). Total RNA was extracted at 6, 12, 18 and 24h timepoint with peqGOLD TOTAL RNA Kit (VWR).