Uncoupling of transcription and cytodifferentiation in mouse spermatocytes with impaired meiosis

We use the cytologically well-defined Prdm9 mutant mouse as a model of developmental arrest to demonstrate that parallel programs of cellular differentiation and transcription can become dis-associated. By comparing cytological phenotype markers and transcriptomes in wild-type and mutant spermatocytes, we identified multiple instances of cellular and transcriptional uncoupling in Prdm9-/- mutants. Overall design: Transcript abundance from mouse spermatocytes with wild-type, heterozygous, and homozygous mutant alleles of Prdm9, collected at three time points with replicates.