RNA sequencing of lung tissue from Fra-2 transgenic mouse (Fra-2tg) and wild-type littermates

Fra-2tg mice develop spontaneous lung fibrosis. We carried out this sequencing with tissue from young animals before they develop the tissue fibrosis, to explore the early gene expression changes that might contribute to the phenotype. Lung tissue from 3 Fra-2tg mice and 3 wild-type littermates (C57BL/6 genetic background) was homogenized and RNA was isolated. After analysis of the RNA quality (RIN>8) in a Bioanalyzer, 1ug of RNA material was provided for the cDNA synthesis and sequencing. The initial objective was to look for differentially expressed genes after comparing Fra-2tg and wild-type groups.