Vanessa cardui, UbxCRE11a2+c5 CRISPR mutant genotyping (G1 generation). Vanessa cardui

Vanessa cardui, laboratory culture 2022-2023 in the Martin Lab at the George Washington University.Stock Origin, Carolina Biological Supplies.Syncytial embryos where microinjected with Cas9-2xNLS targeting the Ubx cis-regulatory module CRM11:UbxCRE11a2_sgRNA CTACTCTGTTCGGACATTCG;UbxCRE11c5_sgRNA GCTGCCGCGAGTCTGAATCG.G1 individuals were obtained from in-crossing G0 crispants and genotyped on a Novaseq S4 P4 2x150 PE library.Study in preparation:The cis-regulatory architecture of the Ultrabithorax locus in developing butterfly wings.Amruta Tendolkar 1, Anyi Mazo-Vargas 1, Luca Livraghi 1, Joseph J. Hanly 1,2, Kelsey Van Horne 1, Lawrence E. Gilbert 3, Arnaud Martin 11: Department of Biological Sciences, The George Washington University, Washington, DC, United States.2: Smithsonian Tropical Research Institute, Panama.3: Department of Integrative Biology, University of Texas, Austin, Austin, TX, United States.