Single-cell RNA sequencing of blood cells from patients with VEXAS syndrome.

We performed single cell RNA sequencing, and VDJ sequencing of TCR and BCR of peripheral blood samples (Peripheral Blood Mononuclear Cells[PBMCs]) from 9 patients with VEXAS to understand disease pathogenesis. RNA profiles of single peripheral blood cells of 9 VEXAS patients and 5 healthy donors were generated by deep sequencing using Illumina NovaSeq.