HCQ blocks ERK-dependent dephosphorylating of BAD and reduces cleaved caspase 9 in neonatal rat cardiomyocytes in simulated I/R injury.

Western blot analysis of cell lysates from neonatal rat cardiomyocytes pre-treated overnight with 2000 ng/mL HCQ and the following day exposed to simulated I/R injury (4h hypoxia + 2h reoxygenation). BAD dephosphorylating at serine 112 (A) and serine 136 (B) and levels of cleaved caspase-8 and caspase-9 were assessed using western blot. Graphs show mean ±SEM of quantitative analysis from three independent experiments. Statistical analysis determined by 1 way ANOVA using post-hoc Tukey to compare all columns (*p<0.05, ** p<0.005, *** p<0.0005).