Lysis solutions were assessed in protocols aimed at developing a simplified extraction method.

Shown are the protocols and reagents with their respective concentrations and volumes necessary for the preparation of lysis solutions evaluated in DNA extraction from 3 mm skin fragment of swine skin; *PBS: Phosphate Buffered Saline; NH4OH: ammonium hydroxide; Tx100: Triton X-100; SDS: Sodium Dodecyl Sulfate. Initially, all solutions were tested using porcine skin as the experimental model. Following the identification of the most effective lysis solution, which was solution 2, we proceeded to evaluate using skin biopsies from human patients.
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