Endothelial CEPT1 Promotes Angiogenesis Through PPAR?? and VEGF-A Signaling

Cept1 is essential for de novo phopholipogenesis and is impacted by diabetes. We previously demonstrated that conditional knockdown of Cept1 in the endothelium leads to reduced tissue recovery. Therefore, we hypothesized that Cept1 may also be sufficient in promoting post-ischemic angiogenesis and recovery in the setting of diabetes. We have employed a single cell sequencing approach using 10x Genomics scRNAseq to study the role of Cept1 overexpression in mice aortic tissue. Overall design: An endothelial cell (EC)-specific Cept1 overexpression mouse model was developed (Cept1fl/flCre+) in adult C57BL6 mice. Murine aortas were harvested, for single-cell RNA sequencing (scRNA-seq). Transcriptional profiling of Cept1fl/fl Cre- and Cept1fl/fl Cre+ mouse whole aortas was performed using droplet-based massively parallel 10X genomic single-cell RNA sequencing (scRNA-seq) 3' v3.