Sample preparation strategies for HMGB1

Investigating the outcomes of antibody-free sample preparation strategies for LC-MS/MS analysis of high mobility group 1 (HMGB1) protein in human plasma. The number of peptides and other proteins identified was found by searching the Global Proteome Machine (GPM) database using these Mascot generic format-files.本研究探究了无抗体样品前处理策略应用于人血浆中高迁移率族蛋白1（high mobility group 1, HMGB1）的液相色谱-串联质谱（LC-MS/MS）分析效果。通过使用Mascot通用格式文件（Mascot generic format-files）检索全球蛋白质组学机器（Global Proteome Machine, GPM）数据库，确定了本次实验中鉴定得到的肽段与其他蛋白质的数量。