Tipping point in alpha-synuclein-membrane interactions from stable protein-covered vesicles to amyloid aggregation

HSQC_20C and HSQC_t37C folder contains raw 1H-15N HSQC data acquired on samples containing a-synuclein and SUVs at different lipid-to-protein (L/P) ratios at 20 and 37 degrees C. DiffusionNMR contains diffusion NMR raw data acquired on a-synuclein and SUVs at different L/P ratios at 20 degrees C. The data are analyzed, presented and discussed in Makasewicz et al. (https://doi.org/10.1016/j.xcrp.2024.102309). The folder matlabScript contains all Matlab codes used to simulate NMR spectra, which are shown in the Supplemental Information.HSQC_20C与HSQC_t37C文件夹包含分别在20℃和37℃下，采集自不同脂质与蛋白（L/P）比例的α-突触核蛋白（α-synuclein）与小单层囊泡（SUVs）样品的1H-15N异核单量子相干（HSQC）原始数据。DiffusionNMR文件夹包含采集自20℃下、不同L/P比例的α-突触核蛋白与SUVs样品的扩散NMR原始数据。上述数据的分析、展示与讨论详见Makasewicz等人的研究（https://doi.org/10.1016/j.xcrp.2024.102309）。 matlabScript文件夹内存储了所有用于模拟NMR谱图的Matlab代码，相关细节已在补充材料（Supplemental Information）中展示。