Characterization of Human Bone Marrow Niches with Metabolome and Transcriptome Profiling

The hematopoietic niches in bone marrow (BM) are specialized compartments working in harmony to regulate all the steps of the hematopoiesis cascade. Little is known about how BM niches in healthy humans. Therefore, we investigated the presence of different niches in healthy human BM using comparative omic technologies (Metabolomic and transcriptomic). We sequenced and comparative analyzed mRNA samples of human bone marrow mesenchymal stem cells (MSCs) to generate the gene expression profiles of these cells which obtained from two anatomically different BM regions (R1 and R2). Overall design: In total, 22 human bone marrow-derived mesenchymal stem cells (hBMSC) samples which were obtained from two anatomically different bone marrow region (R1 and R2) of 11 healthy bone marrow transplantation donors analyzed.