Widefield and two-photon recordings of neuronal and vascular changes during single whisker stimulation in the mouse barrel cortex.

These datasets contain recordings of neuronal activity and vascular changes, obtained using widefield optical imaging and two-photon microscopy, following single whisker stimulations in the mouse barrel cortex (vS1). The widefield dataset contains traces and metadata for changes in neuronal Ca2+ activity, changes in oxy-, deoxy- and total hemoglobin concentration (Δ[HbO], Δ[HbR], Δ[HbT] respectively). The two-photon dataset contains traces and metadata for changes in arterioles and capillary diameter, changes in red blood cell velocity and flow, as well as neuronal Ca2+ activity. Mice were either awake or under dexmedetomidine sedation. See Martineau et al (2024) for more details on the experimental design and methods.