ImageJ macro for semi-automated counting of nuclei

This semi-automated nuclei counting method gives the user an opportunity to qualitatively review output results for accuracy. The method was designed to work with fluorescently stained nuclei. If desired, the macro could be modified or applied to other staining methods. Although there are a variety of possible methods to count nuclei (e.g. machine learning), the goal of the method presented here was to run quickly and accurately on a basic consumer-level computer with freely available software (ImageJ).

此半自动核计数方法为用户提供了对输出结果的定性审查以检验准确性的机会。该方法旨在与荧光染色的细胞核协同工作。如需，该宏可以修改或应用于其他染色方法。尽管存在多种可能的核计数方法（例如，机器学习），但本方法所展示的目标是在基本消费级计算机上，使用免费软件（ImageJ）快速且准确地运行。