The microRNA profiles of extracellular vesicles (EVs) from osteoclasts (OCs) co-cultured with prostate cancer (PCa) cell lines [OCEVmiRNA-seq]

Bone metastatic lesions of PCa contain areas of bone destruction and formation that are directed by OCs and osteoblasts (OBs), respectively. OC-derived EVs are reported to maintain normal bone homeostasis; however, there is no study investigating the role of EVs from OCs in the presence of PCa. To investigate the EV contents derived from OCs with PCa cells , we cultured murine monocytic cell line RAW 264.7, which has potential to differentiate into OC-like cells, with metastatic PCa cell lines and controls. We prepared different types of OCs and EVs from these cells ; OCs normally differentiated from RAW264.7 cells (OC cells), OC cells co-cultured with normal prostate epithelial cells: PNT2 cells (OCN cells), osteolytic PCa cells: PC3M cells (OCP cells), and osteoblastic PCa cells; C4-2B cells (OCC cells). OC differentiation was induced in the presence of RANKL. EVs were isolated from culture supernatant using ultracentrifugation.