comparative analysis of the effects of various chamomile extracts and characteristic pure compounds on human T cells

In this study, we have characterized and compared the effects of differently prepared chamomile extracts and characteristic pure compounds on the T cell redox milieu as well as on the migration, activation, proliferation, and cytokine production of primary human T cells. Futhermore, nCounter based gene expression profiling was performed on the most promising extracts (Chamomile aqueous total fermented-CT, aqueous toot fermented-CR, and ethanolic flower-CF) and pure compounds (Apigenin-Ap and Chamazulene-Cz) to identify the genes related to different T cell functions, that were targeted by the different treatment conditions. in this study, total RNA samples were extracted from costimulated T cells of healthy volunteers left unstimulated, or stimulated, each stimulation subjected to a particular treatment condition or not. 4 to 5 healthy donors were taken for each condition, treated or untreated