FIGURE 2 RAW DATA

(A) Increase of
apparent brightnesses of diffusing species. Values of B parameters obtained for
expressions at protein concentrations >150 nM were averaged and normalised
to the sfGFP monomer control. Clearly, the data show that only full-length
MyD88 (red) is capable of forming large complexes, while the TIR domain (green)
or the death domain (blue) form smaller oligomers. Inset: Expansion of the
values obtained for TIR and death domains compared to sfGFP control.

(B) FCS data in
solution. Control based on GFP monomer (black). Correlation curves obtained for
the TIR domain (green), death domain (blue) and full-length MyD88 (red). A
clear shift in diffusion time can be seen between the full-length protein and
the separate domains. FCS curves are
representative traces from 3 repeated measurements.

(C) Hydrodynamic
radius normalised by the sfGFP control (black) calculated for the domains and
full-length MyD88, indicating the increase of approximate physical size of the
oligomeric species. Values are mean ± SD from 3
repeat measurements.